Datasets of relative fluorescence units obtained by spectrofluorometric analysis of filter paper samples and air samples from the environment in a dental clinical setting during simulated dental procedures in a mannequin. Fluorescein was used as a tracer, and an air-turbine or electric micromotor handpiece was used to perform a 10-minuite crown preparation on an upper incisor tooth. Medium volume suction was used concurrently.<div><br></div><div>Datasets of particle counts, temperature, and relative humidity obtained using an optical particle counter under the same conditions.</div><div><br></div><div>Micromotor handpieces were used at three speeds: 60,000 rpm (60K); 120,000 rpm (120K); and 200,000 rpm (200k).</div><div><br></div><div>Air sampling and particle counting data were obtained at three distances from the source: 0.5 m; 1.5 m; 1.7 m.</div><div><br></div><div>Full details of the project can be found at: https://doi.org/10.1016/j.jdent.2021.103746</div>