READ ME This text describes the data presented in the paper: Influence of nerve cuff channel count and implantation site on the separability of afferent ENG ======================== Introductory information ======================== The data was collected from 2 experiments: experiment 1 (Animals 1-4) and experiment 2 (Animals 5-6). For Animals 1-4 a 3- and a 16-channel cuff were simultaneously implanted (acutely) on the sciatic nerve. Mechanical stimulation of the hindpaw included three different types of stimulation: proprioception, nociception and touch. For Animals 5-6 two identical 16-channel cuffs were implanted on the sciatic nerve (one proximally and the other distally). Only proprioception stimulation was applied in experiment 2. Files included in the data deposit (include a short description of what data are contained): 1) .ns5 files The .ns5 file contains the time series data from the electrodes. The sampling rate is 30k/second. NS5.ElectrodesInfo: Animals 1-4 Rows - 1-14, 16 and 18 correspond to the 16-channel cuff electrode Rows - 15, 17 and 19 correspond to the 3-channel cuff electrode RoW 20 - flexsensor (not used) Row 21 - pressure sensor (for synchronisation with the nociception stimuli) RoW 22 - signals to the motors (for knowing the times of proprioception and touch stimuli application) Animals 5 and 6 The even-number rows (1,3...31) correspond to one 16-channel cuff electrode The odd-number rows (2,4...32) correspond to the second 16-channel cuff electrode RoW 20 - flexsensor (not used) Row 21 - pressure sensor (not used) RoW 22 - signals to the motors (for knowing the times of proprioception stimuli application) 2) .nev files The nev files contain the comments and time stamps used for synchronising recordings. The comments are used to know the times of stimulus application. For Animals 1 and 2: Proprioception comments prop_angle_X - moment when the steady-state proprioception begins X can take one of the following six values which correspond to the angle the hindpaw was moved to: 0.667 (30°); 0.333 (-30°); 0.611 (20°); 0.389 (-20°); 0.556 (10°) and 0.444 (-10°) end - end of the steady-state proprioception stimuli Touch stimuli comments 100heel_trial_ (Animal 1) and heel100_trial_ (Animal 2)- moment when the touch stimuli starts (the motors are not moving) end touch - end of touch stimuli ------------------------- For Animals 3-6: Proprioception comments: mb - stands for motor begins moving angle_X_begin - angle (X) at which the hindpaw has been moved to. Steady-state proprioception begins at this point. ep - end of proprioception stimulation, so the motors start moving back to the resting position; me - motor stops moving Touch stimuli comments motor start moving_ - moment when the motor starts moving begin touch - start of the touch stimuli (the motors are not moving) end touch - end of touch stimuli ---------------------------------- ---------------------------------- Both file types can be opened as structures in MATLAB using the openNEV and openNSx functions written by Blackrock microsystems available in the NPMK toolbox. The NPMK toolbox can be downloaded from here https://github.com/BlackrockMicrosystems/NPMK/releases To open the files download NPMK and add to the MATLAB path. Type the command "openNSx" to open the .ns5 file or "openNEV" to open the .nev files into the command prompt. This will open a browser where the file you wish to open can be selected. Double click on the file you wish to open. The file will now appear as a structure title NS5 or NEV in the workspace in MATLAB For NS5 files the structure will contain four fields 1- MetaTags Structure with 18 fields Provides information on how many channels were recorded, the time duration of the recording, sampling frequency etc 2-Data The time series data. Each row corresponds to a recording signal as listed above. The Data is in int8 format and the amplitude in digitised points To change amplitude to uV type the command into the MATLAB command prompt "double(NS5.Data)/4" 3-RawData Structure with 3 fields Contains the headers, DataHeader and information about how long the file was paused for 4-ElectrodesInfo Structure with 16 fields containing information about each of the electrodes used for recording. For NEv files the structure will contain 4 fields 1- MetaTags Structure with 21 fields 2-ElectrodesInfo Structure with 16 fields containing information about each of the electrodes used for recording. 3-Data Structure with 8 fields Contains the TimeStamp comments were added in sample points and in seconds (TimeStamp and TimeStampSec) NEV.Data.Comments.Text contains each comment in the order it was added. 4-IOlabels Explain the relationship between multiple data sets, if required: The .ns5 and .nev files with the same name were recorded at the same time. The comments and timestamps in the .nev file correspond to the time series data in the .ns5 file of the same name. The data is divided into six folders corresponding to the six different animals the data was collected on and labelled with the date they were collected. Each folder contains folders with the type of stimuli collected (proprioception, nociception, touch). Keywords used to describe the data: neural signals sensory signals nerve recordings cuff electrodes ========================== Methodological information ========================== A brief method description – what the data is, how and why it was collected or created, and how it was processed: For a full description of the methodology please see publication Silveira C, Brunton E, Spendiff S, Nazarpour K. Influence of nerve cuff channel count and implantation site on the separability of afferent ENG. 2018 J. Neural Eng. 15 046004. https://doi.org/10.1088/1741-2552/aabca0. The data corresponds to the afferent nerve signals recorded in response to mechanical stimulation of the rats' hindpaw. The nerve signals were digitised and bandpassed filtered (0.3 - 7.5 kHz). The signals were recorded at 30,000 samples per second and further digitally bandpassed filtered (0.8 - 2.2 kHz). Three types of mechanical stimulation were applied and this is indicated in the folders and filenames Proprioception - the foot was moved to 6 angles -30, -20, -10, 10, 20 and 30 degrees. The start and end of the movement are indicated by comments in the NEV file. Nociception - A pinch of the heel or the outer toe. As indicated in the filename. The start and end of the pinch are indicated by the pressure sensor data recorded in row 21 of the .ns5 time series data. Touch - Touch of the middle of the hindpaw with either a 100, 300, 8 or 15 grams Von Frey hair as indicated in the filename. Instruments, hardware, and software used: 16-channel Concentric Nerve cuffs - (Microprobes for Life Science, USA) 3-channel Standard Nerve cuff - (Microprobes for Life Science, USA) Cerebus Neural Signal Processor - (Blackrock Microsystems, USA) Cereplex M-32 head stage - (Blackrock Microsystems, USA) Central Software Suite - (Blackrock Microsystems, USA) MATLAB - (Mathworks, U.S.A) Date(s) of data collection: The data was collected over 6 days from the 02/11/2016 until the 16/05/2017 Data validation (how was the data checked, proofed and cleaned): This is the raw data. Overview of secondary data, if used: ========================= Data-specific information ========================= Definitions of names, labels, acronyms or specialist terminology uses for variables, records and their values: Explanation of weighting and grossing variables: Outline any missing data: ======= Contact ======= Please contact rdm@ncl.ac.uk for further information