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This text describes the data presented in the paper: Key reaction components affect the kinetics and performance robustness of cell-free protein synthesis reactions

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Introductory information
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Files included in the data deposit:

1) Figure 1 Source Data.xlsx 118.24 kB
2) Figure 2A DSD1 design.xlsx 23.04 kB
3) Figure 2A DSD2 design.xlsx 22.97 kB
4) Figure 2A and 2B Source Data.xlsx 247.2 kB
5) Figure 2A DSD1 workflow.json 15.52 kB
6) Figure 2A DSD2 workflow.json 15.48 kB
7) Figure 2D Source Data.xlsx 516.08 kB
8) Figure 2F Source Data.xlsx 121.47 kB
9) Figure 4 Source Data.xlsx 96.71 kB
10) Figure 2E Source Data.xlsx 72.32 kB
11) Figure 3 Source Data.xlsx 108.99 kB
12) Figure 5A and 5B Source Data.xlsx 87 kB
13) Figure 5C Source Data.xlsx 16.73 kB
14) Figure A2 Source Data.xlsx 77.36 kB
15) Figure S2 Source Data.xlsx 89.21 kB
16) Figure A1 Source Data.xlsx 92.33 kB
17) Figure S1 Source Data.xlsx 32.01 kB


These files contain the data underpinning the results and analysis described in the publication.

Key words used to describe the data: cell-free protein synthesis; design of experiments; synthetic biology


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Methodological information
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Cell-free protein synthesis reactions were performed under variable conditions and the impact on kinetics was analysed. Statistical Design of Experiments (screening and Response Surface Modelling) was used to analyse the data and models were subsequently validated by further experimental work.


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Contact
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Please contact rdm@ncl.ac.uk for further information