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This text describes the data presented in the paper:

Spurr MWA, Yu EH, Scott K, Head IM.  No re-calibration required? Stability of a bioelectrochemical organic load sensor over 800 days.

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Introductory information
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Files included in the data deposit (include a short description of what data are contained): 

1) _Batch_Events_data.xlsx
2) _Flow_Events_data.xlsx
3) Batch_AB_136d_GGA_data_export.csv
4) Batch_AB_219d_GGA_data_export.csv
5) Batch_AB_328d_GGA_data_export.csv
6) Batch_AB_730d_GGA_data_export.csv
7) Batch_AB_792d_G+GA+GGA_data_export.csv
8) Batch_CD_56d_G+GA+GGA_data_export.csv
9) Flow_ABC_728d_GGA_data_export.csv
10) Flow_DEF_67d_GGA_data_export.csv
11) I_pk_vals_305.txt
12) I_pk_vals_432.txt
13) I_pk_vals_953.txt
14) I_pk_vals_5100.txt
15) Rext_data_export.csv
16) Rext_BatchCalib.txt
17) Rext_CE_RESPTIME.xlsx


Explain the relationship between multiple data sets, if required:

_Batch_Events_data.xlsx contains the timestamped events data in the following tabs pertaining to batch-mode MFCs and OCP controls.
'Batch_AB' tab for batch-mode MFCs A & B (+ OCP A, B, C)
'Batch_CD' tab for batch-mode MFCs C & D
- Column Batch.Days = Time in days since inoculation
- Calibration = ID of calibration medium change belongs to
- New.Med. = 0 = Medium replacement
- Med.Type. = Medium carbon source type
- Sub.Change = 0 = substrate type changed
- Op.Mode = Operation mode for pump
- Op.Change = 0 indicating op.mode change
- Prep.Vol = Prepared volume of medium in ml
- Med.Conc = Medium Concentration in mg/l
- Rex.Change = Resistor replaced
- F1G1.Rex = Value of RExt
- OCP. = 0 indicates resistor disconnected (in OCP)
- Pol.Curve = 0 indicates polarisation curve conducted
- PC.Rex = 0 indicates resistor value is part of polarisation curve not operation
- GDE.Rep = 0 indicates Gas Diffusion Electrode + membrane replaced
- RE.Rep = 0 indicates Ag/AgCl reference electrode replaced
- Setup.Change = See comments
- Cells.Online = Indicates cell status
- Comments

_Flow_Events_data.xlsx contains the timestamped events data in the following tabs pertaining to flow-mode MFCs.
'Flow_ABC' tab for flow-mode MFCs A1, A2, A3, B1, B2, B3, C1, C2 & C3
'Flow_DEF' tab for flow-mode MFCs D1, D2, D3, E1, E2, E3, F1, F2 & F3
- Column Flow.Days = Time in days since inoculation
- Calibration = ID of calibration medium change belongs to
- New.Med. = 0 = Medium replacement
- Med.Type. = Medium carbon source type
- Sub.Change = 0 = substrate type changed
- Op.Mode = Operation mode for pump
- Op.Change = 0 indicating op.mode change
- Prep.Vol = Prepared volume of medium in ml
- Med.Conc = Medium Concentration in mg/l
- Pump.Event. = 0 indicates Pump changed
- Pump.On. = Pump status
- RPM = Pump speed in rpm
- Emptied.Waste.Bottle. = 0 indicates replacement.
- Rex.Change = Resistor replaced
- *.Rex = Value of RExt
- OCP. = 0 indicates resistor disconnected (in OCP)
- Pol.Curve = 0 indicates polarisation curve conducted
- PC.Rex = 0 indicates resistor value is part of polarisation curve not operation
- GDE.Rep = 0 indicates Gas Diffusion Electrode + membrane replaced
- RE.Rep = 0 indicates Ag/AgCl reference electrode replaced
- Setup.Change = See comments
- Sludge.Removal = Sludge removed by flushing MFCs at high flow rate and cleaning tubing.
- Cells.Online = Indicates cell status
- Comments

Batch_AB-denoted files represent data for batch-mode MFCs A & B
Batch_AB-denoted files represent data for batch-mode MFCs C & D
Flow_ABC-denoted files represent data for flow-mode MFCs in the ABC series
Flow_DEF-denoted files represent data for flow-mode MFCs in the DEF series

Files suffixed *data_export.csv columns:
- Time.d = Timestamp (in days since Time zero)
- Stage = Batch or MFC Stage (1,2 or 3) and Sum (S)
- For replicates A, B (and C) as 'X':
- R_mean is the mean of A, B (and C) values
- R_range is the standard deviation of A, B (and C) values
-- X.mV = Recorded voltage in mV
-- X.An = Recorded anode potential in mV
-- X.j = Calculated current in mA
-- A.Q = Calculated charge in C

Files prefixed I_pk_vals_*.txt with * representing RExt used for calibration plots, columns:
- Calibration = Calibration series ID
- RExt = Resistance used
- DateTime = Timestamp
- Med.Conc = Medium concentration in mg/l
- Med.Type = Medium carbon source type
- BOD5 = Calculated BOD5 in mg/l
- Stage = Batch or MFC Stage (1,2 or 3) and Sum (S)
- A/B/C = Replicate current density (uA/cm2)
- R_mean = Mean response of replicates (uA/cm2)
- R_range/R_error = Range (duplicates) or standard deviation (triplicates) of mean (uA/cm2)
- *max = Maximum value used for normalisation
- An/Bn/Cn = Normalised current densities
- R_meann = Normalised mean current (%)
- R_rangen = Normalised error (%)

Rext_BatchCalib.txt columns:
- Calibration = Calibration series ID
- Name = row ID
- Date.st = Date calibration started
- Date.en = Date calibration ended
- Med.Conc = Medium concentration in mg/l
- Med.Type = Medium carbon source type

Rext_CE_RESPTIME.xlsx columns:
43.2, 305, 953, 5100 tabs:
- id = row ID
- Calibration = Calibration series
- Q.Cyc = cycle id
- R_mean = Mean current density (uA/cm2)
- R_range = Range current density of duplicates (uA/cm2)
- Med.Conc = Concentration of medium (mg/l)
- Coulombic Efficiency = Mean (%)
- Range = Range (%)
- Included = Included in grand mean at bottom of table

RespTime tab:
At the bottom of each table for each resistance the mean response time to peak in hours is calculated alongside standard deviation.


Key words used to describe the data:

Microbial Fuel Cell, Biochemical oxygen demand, Toxicity, Biosensor

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Methodological information
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A brief method description – what the data is, how and why it was collected or created, and how it was processed:

Data obtained from operation and calibration of multi-stage Microbial Fuel Cells (voltage datalogging, medium replacements).

Instruments, hardware and software used: 

Microbial Fuel cell reactors were manufactured in-house at Newcastle University.

National Instruments USB-6225 datalogger was used to monitor MFC voltages.
National Instruments SignalExpress datalogging software

Dissolved oxygen (for BOD5 measurements) was measured with a Hach HQ40d meter

Date(s) of data collection: 

2012-2015

Geographic coverage of data:

Experiments undertaken in Newcastle upon Tyne, UK.

Data validation (how was the data checked, proofed and cleaned):

Batch-mode MFCs were operated in duplicate (A & B, or, C & D) to validate results.
Flow-mode MFCs were operated in triplicate (A, B & C, or, D, E & F) to validate results.

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Data-specific information
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Definitions of names, labels, acronyms or specialist terminology uses for variables, records and their values: 

A - Batch reactor A
B - Batch reactor B
C - Batch reactor C
D - Batch reactor D

1A - 1st MFC in tubular reactor A
2A - 2nd MFC in tubular reactor A
3A - 3rd MFC in tubular reactor A

1B - 1st MFC in tubular reactor B
2B - 2nd MFC in tubular reactor B
3B - 3rd MFC in tubular reactor B

1C - 1st MFC in tubular reactor C
2C - 2nd MFC in tubular reactor C
3C - 3rd MFC in tubular reactor C

1D - 1st MFC in tubular reactor D
2D - 2nd MFC in tubular reactor D
3D - 3rd MFC in tubular reactor D

1E - 1st MFC in tubular reactor E
2E - 2nd MFC in tubular reactor E
3E - 3rd MFC in tubular reactor E

1F - 1st MFC in tubular reactor F
2F - 2nd MFC in tubular reactor F
3F - 3rd MFC in tubular reactor F

Explanation of weighting and grossing variables: 

Where applicable the mean and standard deviation of triplicated data was taken.

Outline any missing data:

N/A

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Contact
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Please contact rdm@ncl.ac.uk for further information