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This text describes the data presented in the paper:

Spurr MWA, Yu EH, Scott K, Head IM.  A microbial fuel cell sensor for unambiguous measurement of organic loading and definitive identification of toxic influents. Environ. Sci. Water Res. Technol. 2020, 6, 612-621.


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Introductory information
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Files included in the data deposit (include a short description of what data are contained): 

1) BOD_increase_data.xlsx
2) LoBODSummary.txt
3) LoBODEventStatus.txt
4) LoBODSamples.txt
5) 4NPSummary.txt
6) 4NPEventStatus.txt
7) 4NPSamples.txt
8) BODIncSummary.txt
9) BODIncEventStatus.txt
10) SubInhSummary.txt
11) SubInhEventStatus.txt


Explain the relationship between multiple data sets, if required:

BOD_increase_data.xlsx contains the main data in the following tabs pertaining to Figures 2&3.
'Calibrations' tab
- Column Calibration = id
- Column Time.st = Start time of calibration in days
- Column Time.en = End time of calibration in days
- Column Med.Conc = Medium concentration in mg/l
- Column Med.Type = Medium carbon source type

'Events' tab
- Column Time.d = Timestamp (in days since Time zero)
- Cells = Cells concerned with event row
- Calibration = Calibration id
- New.Med. = 0 Indicating medium was replaced
- Med.Type. = Medium carbon source type
- Op.Mode = Operation mode for pump
- Op.Change = 0 indicating op.mode change
- Prep.Vol = Prepared volume of medium in ml
- Med.Conc = Medium Concentration in mg/l
- N2.Sparge. = 0 indicating N2 sparge occurred
- N2.Sparge.Time = Inidicating ON/OFF for N2 sparge of medium
- Pump.Event = 0 indicating pump event
- Pump.On = Pump status
- RPM = Pump speed
- Emptied.Waste.Bottle = 0 indicates event
- Setup.Change = See comments
- Sludge.Removal = 0 indicates event
- Cells.Online = Indicates cell status
- Comments

'Data_MFCs' tab
- Time.d = Timestamp (in days since Time zero)
- Stage = MFC Stage (1,2 or 3) and Sum (S)
- For replicates A, B and C as 'X':
- R_mean is the mean of A, B and C values
- R_range is the standard deviation of A, B and C values
-- X.mV = Recorded voltage in mV
-- X.An = Recorded anode potential in mV
-- X.j = Calculated current in mA
-- A.Q = Calculated charge in C

LoBOD-denoted files represent data for BOD decrease events depicted in Figure 4&5
4NP-denoted files represent data for 4-Nitrophenol toxicity events depicted in Figure 6&7
SubInh-denoted files represent data for Substrate Inhibition events depicted in Figure 8
BODInc-denoted files represent data for BOD Increase events depicted in Figure 8

Files suffixed *Summary.txt columns:
- id = Row ID
- Time.h = Timestamp (in hours since Time zero)
- LoBOD/4NP/SubInh/BODInc = Concentration of parameter in mg/l
- Stage = MFC Stage (1,2 or 3) and Sum (S)
- A/B/C = Normalised current (current/max current) in (%)

Files suffixed *EventStatus.txt columns:
- ID = Row ID
- Time.h.s = Start time of event in hours
- Time.h.e = End time of event in hours
- Stage = MFC Stage (1,2 or 3) and Sum (S)
- Tox.ev = 0 representing event occurred.

Files suffixed *Samples.txt columns:
- id = Row ID
- LoBOD/4NP = Concentration of parameter in mg/l
- Stage = Location COD measured from (M) Medium, (A,B,C) MFC effluent
- COD = Mean concentration of COD measured
- SD = Standard deviation of COD measured

Key words used to describe the data:

Microbial Fuel Cell, Biochemical oxygen demand, Toxicity, Biosensor

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Methodological information
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A brief method description – what the data is, how and why it was collected or created, and how it was processed:

Data obtained from operation and calibration of multi-stage Microbial Fuel Cells (voltage datalogging, medium replacements).

Instruments, hardware and software used: 

Microbial Fuel cell reactors were manufactured in-house at Newcastle University.

National Instruments USB-6225 datalogger was used to monitor MFC voltages.
National Instruments SignalExpress datalogging software

Dissolved oxygen (for BOD5 measurements) was measured with a Hach HQ40d meter

COD was measured using Merck kits.
4-NP was measured using a Spectroquant Pharo 300 spectrophotometer.

Date(s) of data collection: 

2012-2015

Geographic coverage of data:

Experiments undertaken in Newcastle upon Tyne, UK.

Data validation (how was the data checked, proofed and cleaned):

MFCs were operated in triplicate (A, B & C) to validate results.

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Data-specific information
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Definitions of names, labels, acronyms or specialist terminology uses for variables, records and their values: 

1A - 1st MFC in tubular reactor A
2A - 2nd MFC in tubular reactor A
3A - 3rd MFC in tubular reactor A

1B - 1st MFC in tubular reactor B
2B - 2nd MFC in tubular reactor B
3B - 3rd MFC in tubular reactor B

1C - 1st MFC in tubular reactor C
2C - 2nd MFC in tubular reactor C
3C - 3rd MFC in tubular reactor C

Explanation of weighting and grossing variables: 

Where applicable the mean and standard deviation of triplicated data was taken.

Outline any missing data:

N/A

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Contact
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Please contact rdm@ncl.ac.uk for further information