READ ME This text describes the experimental data presented in the paper: Temporal Modulation of the Response of Sensory Fibers to Paired-Pulse Stimulation. ======================== Introductory information ======================== Files included in the data deposit (include a short description of what data are contained): The data is included for 4 animals. Animal 1 - was recorded from on the 19/12/2017 Animal 2 - was recorded from on 19/04/2018 Animal 3 - was recorded from on the 26/04/2018 Animal 4 - was recorded from on the 31/05/2018 1) .ns5 files The .ns5 file contains the time series data from the electrodes. The sampling rate is 30k/second. Row 1 - 2 Bipolar recording from tungsten wires place on the L4 Dorsal Root (Sometimes one of these was disconnected.) rOW 3 - Bipolar EMG from the Tibialis Anterior muscle Row 4 - Synchronisation signal, a rising edge indicates the initiation of the stimulus 2) .nev files The nev file contains the comments and time stamps used for synchronising recordings. Both file types can be opened as structures in MATLAB using the openNEV and openNSx functions written by Blackrock microsystems available in the NPMK toolbox. The NPMK toolbox can be downloaded from here https://github.com/BlackrockMicrosystems/NPMK/releases To open the files download NPMK and add to the MATLAB path. Type the command "openNSx" to open the .ns5 file or "openNEV" to open the .nev files into the command prompt. This will open a browser where the file you wish to open can be selected. Double click on the file you wish to open. The file will now appear as a structure title NS5 or NEV in the workspace in MATLAB For NS5 files the structure will contain four fields 1- MetaTags Structure with 17 fields Provides information on how many channels were recorded, the time duration of the recording, sampling frequency etc 2-Data The time series data. Each row corresponds to a recording signals as listed above. The Data is in int8 format and the amplitude in digitised points To change amplitude to uV type the command into the MATLAB command prompt "double(NS5.Data)/4" 3-RawData Structure with 3 fields Contains the headers, DataHeader and information about how long the file was paused for 4-ElectrodesInfo Structure with 16 fields containing information about each of the electrodes used for recording. For NEv files the structure will contain 4 fields 1- MetaTags Structure with 21 fields 2-ElectrodesInfo Structure with 16 fields containing information about each of the electrodes used for recording. 3-Data Structure with 8 fields Contains the TimeStamp comments were added in sample points and in seconds (TimeStamp and TimeStampSec) NEV.Data.Comments.Text contains each comment int the order it was added. 4-IOlabels Explain the relationship between multiple data sets, if required: The .ns5 and .nev files with the same name were recorded at the same time. The comments and timestamps in the .nev file correspond to the time series data in the .ns5 file of the same name. The data is divided into three folders corresponding three different animals the data was collected on, and labelled with the date they were collected. Key words used to describe the data: neural signals sensory response nerve recordings cuff electrodes ========================== Methodological information ========================== A brief method description – what the data is, how and why it was collected or created, and how it was processed: For a full description of the methodology please see publication Brunton E, Silveira C, Roswnber J, Schiefer M, Riddell J, Nazarpour K. Temporal Modulation of the Response of Sensory Fibers to Paired-Pulse Stimulation. The data is the nerve signals recorded from the L4 Dorsal Root and EMG from the Tibialis anterior muscle in response to stimulation by a cuff electrode on the sciatic. The nerve signals were digitised and bandpassed filtered (10 - 5000 Hz). The signals were recorded at 30,000 samples per second. Electrical Stimulation was applied to the sciatic nerve from a nerve cuff the current return was placed in the skin above the nerve. Electrical Stimulation was applied in two parts. Part 1 - A single pulse was applied to a single electrode to find the threshold required to generate a compound action potential recorded from the dorsal root. This was repeated 10 times in each file. Part 2 - Paired pulse paradigm was applied where the first pulse was applied from one electrode and the second pulse was applied from a different electrode. The timing between each pulse was varied from 0 to 5 ms.This was repeated 10times in each file Instruments, hardware and software used: Concentric Nerve cuff - (Microprobes for Life Science, USA) Cerebus Neural Signal Processpor - (Blackrock Microsystems, USA) Cereplex M-32 head stage - (Blackrock Microsystems, USA) Central Software Suite - (Blackrock Microsystems, USA) MATLAB - (Mathworks, U.S.A) Date(s) of data collection: The data was collected over 1 day on the 27/10/2017 Data validation (how was the data checked, proofed and cleaned): This is the raw data. Overview of secondary data, if used: ========================= Data-specific information ========================= Explanation of filenames part 1 - single pulse Filename will begin with elec, the number following elec indicates the channel that was stimulated, there were 16 channels, only the even channels were connected. The number following I indicates the current that was used for stimulation The number following RE indicates the number of times the stimulation paradigm was repeated in that file The number following p indicates the percent of threhold that was used in the case where the stimulation was repeated 10 times. part 2 - paired pulse File name will begin with CH1, the number following CH1 indicates the channel that was used to send the first pulse in the pair stimulus. The number following CH2 indicates the channel that was used to send the second pulse in the paired stimulus The number following interval indicates the time between the two pulse in ms. ======= Contact ======= Please contact rdm@ncl.ac.uk for further information