READ ME This text describes the data measured at Newcastle University and presented in the paper: Organic carbon amendments for enhanced biological attenuation of trace organic contaminants in biochar-amended stormwater biofilters, Environ. Sci. Technol., 2017, DOI: 10.1021/acs.est.7b01164 ======================== Introductory information ======================== Files included in the data deposit (include a short description of what data are contained): 1) qPCR.xls contains the numerical data underpinning the gene copy numbers measured 2) Bridget.fastq contains the raw gene sequencing data 3) Bridgetmap.txt explains the primers used for each sample Explain the relationship between multiple data sets, if required: Key words used to describe the data: Averages and standard deviations (St.Dv) ========================== Methodological information ========================== A brief method description – what the data is, how and why it was collected or created, and how it was processed: Gene sequencing and quantification data, see analytical method description in the paper. Instruments, hardware and software used: PCR reactions were conducted using the FastStart High Fidelity PCR System and the PCR Nucleotide Mix (Roche Diagnostics GmbH) Sequencing was carried out with an Ion Torrent Personal Genome Machine (PGM) System (Life Technology) at Newcastle University Date(s) of data collection: 01/01/2014 - 31/12/2016 Geographic coverage of data: UK Data validation (how was the data checked, proofed and cleaned): Sequencing raw data has been deposited, see method statement in the paper for the data processing. For the qPCR data, serial dilutions were run, and only the 100 times dilution data is deposited, because lesser dilutions showed PCR inhibition Overview of secondary data, if used: ========================= Data-specific information ========================= Definitions of names, labels, acronyms or specialist terminology uses for variables, records and their values: See Bridgetmap.txt Explanation of weighting and grossing variables: Outline any missing data: Data obtained at the Colorado School of Mines has not been deposited in this repository. Please contact the corresponding author of the publication for queries related to that data. ======= Contact ======= Please contact rdm@ncl.ac.uk for further information