READ ME

This text describes the data presented in the paper: <Temporary Single-Cell Coating for Bioprocessing with a Cationic Polymer> 

========================
Introductory information
========================
Files included in the data deposit (include a short description of what data are contained): 

1) Caspase-3 activity and membrane permeability assay (Analysis of cell viability);
2) Cell aggregation and bioprinting (Here, cell agglomerate images and area, as well as, bioprinting data is presented);
3) Cell Karyotype (Cell karyotyping images);
4) Confocal microscopy (Confocal microscopy pictures for the different cells and polymer concentrations)
5) FACS data (FACS data for the different cells and polymer concentrations)
6) Live-Dead data (Viability data for the different cells and polymer concentrations)
7) MTT (Cell metabolism data for the different cells and polymer concentrations)
8) Transmission Electron Microscopy (Transmission Electron Microscopy data for the different cells and polymer concentrations).



Explain the relationship between multiple data sets, if required:
N/A


Key words used to describe the data:
bioprinting; bioprocessing; cellular uptake; coating; polycation; single cells; temporary


========================== 
Methodological information
==========================
A brief method description – what the data is, how and why it was collected or created, and how it was processed:
All the experimental data obtained and included in the manuscript "Temporary Single-Cell Coating for Bioprocessing with a Cationic Polymer" is presented. 
Here, a single cell speckled coating process was used to stabilize inkjet cell printing bio-inks in order to increase the system's reliability. 
Caspase-3 activity and membrane permeability assays, cell aggregation and bioprinting, cell karyotype, confocal microscopy, FACS, Live-Dead, 
MTT and transmission electron microscopy data is provided. The data obtained was processed using the recommended softwares.


Instruments, hardware and software used: 
ImageStream X Mark II Imaging Flow Cytometer (Amnis); IDEAS software (Merck Millipore); Spectrophotometer (Sunrise, Tecan); Leica DM IL LED, Leica Microsystems;
SPOT Advanced software (SPOT Imaging Solutions); Leica TCS SP2 UV AOBS MP (Upright); Phillips CM 100 Compustage (FEI) transmission electron microscope (Philips); 
FACSCalibur flow cytometer (BD Biosciences); FACS - Flowing Software 2.5.1; ImageJ (National Institutes of Health); Jetlab 4 (Microfab Technologies, Inc.); 
Inverted microscope (Leica DM IL LED, Leica Microsystems)


Date(s) of data collection: 
Between 01/2015 and 03/2017


Geographic coverage of data:
United Kingdom


Data validation (how was the data checked, proofed and cleaned):
Three independent experiments of triplicates per group

Overview of secondary data, if used:
N/A

=========================
Data-specific information
=========================
Definitions of names, labels, acronyms or specialist terminology uses for variables, records and their values: 
Neo-NHDF - Normal Neo-natal Human Dermal Fibroblast; TC-71 - Ewing's Sarcoma; U2OS - Osteosarcoma; PLL - Poly-L-Lysine.



Explanation of weighting and grossing variables: 

N/A

Outline any missing data:

N/A

=======
Contact
=======
Please contact rdm@ncl.ac.uk for further information