Manuscript: Intra-articular injection of autologous tolerogenic dendritic cells modifies the synovial immune landscape in rheumatoid and inflammatory arthritis
Data Repository: Tolerogenic Dendritic Cell Effects on Synovial Immune Landscape
This collection contains data associated with the study investigating how intra-articular tolerogenic dendritic cells (tolDC) affect the local synovial immune landscape in autoimmune arthritis. This work builds upon our previously reported trial where high doses of tolDC appeared to stabilize knee symptoms, and specifically examines the synovial tissue changes using multi-parametric imaging mass cytometry (IMC).
The study analyzed paired synovial biopsies from 7 participants (5 tolDC treated and 2 control) taken at baseline and 14 days after intra-articular injection. Using a 27-antibody IMC panel defining lymphoid, myeloid, and stromal cells, we identified a significant tolDC-induced increase in synovial myeloid cells expressing high levels of the inflammation resolution marker Mer tyrosine kinase (MerTK-High), which correlated with tolDC dose and inversely correlated with arthroscopic synovitis scores.
Repository Contents
Project Documentation
- README file with complete file descriptions
Raw Data
- Individual MCD files from imaging mass cytometry acquisition
- Donor ROI information - Metadata for each donor's regions of interest (ROI), including diagnosis and treatment received, with anonymized donor identification traceable to the original AuToDeCRA study (doi: 10.1136/annrheumdis-2015-208456)
Processed Data
- FCS files and analysis files (pre- and post-transformation)
- DNS format processed files
Analysis and Visualization
- Manuscript figures in GraphPad Prism format
- Cluster percentage data
- Spatial neighborhood analysis data
- Spatial interaction heatmaps
Validation Materials
- Antibody immunofluorescence validation images
