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MolMet Figure 6: Higher blood cholesterol and lower fasting blood glucose and insulin in P446L mice on high energy diets.

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posted on 2023-04-19, 15:49 authored by Brian FordBrian Ford, Loranne AgiusLoranne Agius

A-F) Chronic study of P446L mice on HFD: A. Experimental design.

B) Body weight, liver weight and epididymis fat pad weight, (n=7,7).

C) No difference in free-feeding blood glucose and insulin (21 wk, n=7,7) or glucose tolerance (4-wk, GTT, n=9,9).

D) Higher blood cholesterol and liver triglyceride by LL genotype but no difference in blood triglyceride or liver cholesterol (n=6-7).

E) Representative hematoxylin-eosin images showing microvesicular steatosis in LL-genotype.

F) Histopathology scores for steatosis, microvesicular steatosis, lipogranulomas, lobular Inflammation, portal Inflammation and fibrosis showing higher microvesicular steatosis by LL genotype (n=7,7).

G-L) Chronic study of P446L mice on HFHSD: G. Experimental design. H. Body weight, liver weight and higher epididymal fat pad wt by LL genotype, n=11-13.

I) Free feeding blood glucose and insulin and glucose tolerance test (GTT), n=11-14.

J) Lower post-prandial blood glucose (2h food withdrawal) and insulin and inorganic phosphate (Pi) at cull, n=10-14.

K) Higher blood cholesterol in LL genotype but no difference in blood triglycerides or liver triglycerides and cholesterol, n=10-14.

L) Histopathology scores showing higher lipogranuloma and lower fibrosis scores by LL genotype, n=14,13.

M) Representative hematoxylin-eosin images showing lipogranulomas and Sirius red fast green (SRFG) for fibrosis staining.

Categorical data statistical differences was by Chi Square test; other analysis was by t-test #P< 0.05; ##P< 0.01; ### P<0.001 for genotype effect.

Funding

MICA: Exploring a new perspective on the mechanism of action of Glucokinase Activators in liver, a preclinical study

Medical Research Council

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