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File comprising the raw values used to create the figures present in the publication "Bioinspired Collagen/Hyaluronic Acid/Fibrin-Based Hydrogels for Soft Tissue Engineering: Design, Synthesis, and In Vitro Characterization"

dataset
posted on 2023-10-18, 10:18 authored by Bianca Bindi, Annalisa Perioli, Priscila MeloPriscila Melo, Clara Mattu, Ana Ferreira-DuarteAna Ferreira-Duarte

The dataset submitted contains the raw analytical data, after being treated, used to create the graphs for the following analysis:

  • Figure 2b - degree of Crosslinking obtained from the fluorescence measurements after a TNBS assay which assessed the amount of free amines in a molecule, in this case, collagen. Values were then reported as percentage of free amines.
  • Figure 2c - Rheological data showing the viscoelastic properties of the produced hydrogel materials, depending on the ratio and concentration of crosslinker, PEG.
  • Figure 2d - Rheological data showing the viscosity variation according to different shear rates to demonstrate how the material deforms and assess its suitability for bioprinting.
  • Figure 3 - Rheological data showing the viscoelastic properties of the selected hydrogel formulation, CHAF_1, with time (a), upon different strains (deformation) (b), and stiffness (c).
  • Figure 4b - data collected from the spectroscopy analysis (FTIR) on the raw materials and final hydrogel to study the molecular conformation.
  • Figure 5b - Measurement of how much water is taken by the dry material, based on weight changes, converted into percentage.
  • Figure 6b - Values of pore diameter obtained from manual measurement of pores from images obtained via scanning electron microscopy. Data is arranged as a distribution graph.
  • Figure 6d - Measurement of how much weight is loss for the samples immersed in physiological conditions ( weight changes converted into percentage).
  • Figure 7b - Fluorescence measurement of the data obtained from a presto blue assay, which studies the metabolic activity of the cell-seeded onto the hydrogel and control plastic.
  • Figure 8a,b - Fluorescence measurement of the data obtained from a presto blue assay, which studies the metabolic activity of the cell-seeded into the hydrogel and control plastic.
  • Figure 9b - Values obtained for the area of spheroids measured from micrographs taken with an optical microscope. Area monitored across 7 days.
  • Figure 9d - Cell viability using a measurement of green fluorescence.

Funding

EPSRC RiHN (Redistributed Manufacturing in Deployed Medical Care Network Plus), grant number EP/T014970/1.

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