Figure 1 source data

This dataset is part of the 'Source data and representative raw data for "Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis"' collection. For a link to the related paper, study-level information and guide to the data, as well as viewing all other related research data, please visit the collection.

This data is associated with Figure 1 of the paper in Nature Microbiology. It contains videos of vertically trapped Bacillus subtilis cells expressing fluorescent proteins FtsZ and PBP2B, showing single-molecule tracking of the latter around the division ring. It also contains data from further down the processing and analysis pipeline from the same videos.

220303_slide2_5_MMStack_Default_ring34.ome corresponds to Fig. 1b, left panels.

220309_6_MMStack_Default_ring5.ome corresponds to Fig. 1b, right panels.

220316_5_MMStack_Default_561_denoise.ome_ring15 corresponds to Fig. 1c, top kymograph.

220316_5_MMStack_Default_561_denoise.ome_ring14 corresponds to Fig. 1c, bottom kymograph.

220412_slide2_6_MMStack_Default_561_denoise.ome_ring11 corresponds to Fig. 1d, top kymograph.

220414_4_MMStack_Default_561_denoise.ome_ring3 corresponds to Fig. 1d, bottom kymograph.

220809_sh147_phmm_30c_vercini_fosfomycin_slide2_006_561_denoise_ring1 corresponds to Fig. 1e, top kymograph.

220809_sh147_phmm_30c_vercini_fosfomycin_slide2_001_561_denoise_ring8 corresponds to Fig. 1e, bottom kymograph.

230613_sh147_speeds_phmm30_gauss_2nmsbin and 230605_sh147_speeds_phmm30_logscale correspond to Fig. 1f.

230614_sh147_phmm30_penG_2foldedgauss_2nmsbin and 230605_sh147_speeds_phmm30_penG_logscale correspond to Fig. 1g.

230614_sh147_phmm30_fosfomycin_2foldedgauss_2nmsbin and 230605_sh147_speeds_phmm30_fos_logscale correspond to Fig. 1h.

Whitley_2024_Fig1_SourceData contains all the data used to produce histograms in Fig. 1f, 1g, and 1h.