BP Figure 6: AZD1656 increased liver GK activity in the Gckr^del/wt mouse.

Gckr^wt/wt and Gckr^del/wt were fed on a HFD without (open bar) or with (shaded bar) 3 mg/kg AZD1656 for 16 wk. A) Representative liver GKRP immunostaining of Gckr^wt/wt, Gckr^del/wt and Gckr^del/del genotypes on regular diet. B) Body weight after 16 wk on HFD -/+ AZD1656. C) Representative liver immunostaining for GKRP and GK at the end of the study on HFD -/+ 3 AZD1656. D) GKRP N-H scores; GK N-H scores, GK C-H scores and GK N-H/C-H ratio showing lower GKRP and GK nuclear staining and greater cytoplasmic GK distribution by del/wt genotype. E) GKRP-immunoactivity by Western blot showing more modest GKRP deficiency in del/wt genotype (30%) than in LL (80%) genotype. F) GKRP and GK immunoactivity after 16 wk study in Gckr^wt/wt showing an increase in GK / GKRP immunoactivity ratio by AZD1656 treatment. G) GKRP and GK immunoactivity after 16 wk study in Gckr^del/wt mice showing an increase in GK and GK / GKRP immunoactivity ratio by AZD1656 treatment. H) Lower liver GK activity in the Gckrdel/wt mice and increase by AZD1656 treatment. *P < 0.05 effect of AZD1656; #P < 0.05 effect of genotype.